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Table 2 Methylcitrate synthase activity in cell extracts of yeast and mycelial forms of P. lutzii incubated in glucose, glucose with propionate or only propionate

From: Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses

 ConditionMCS (SD)MCS (SD) Dephosphorylated
YeastGlucose0.510 (±0.005)0.224 (±0.002)
Glucose/Propionate0.708 (±0.005)0.307 (±0.004)
Propionate0.952 (±0.009)0.391 (±0.008)
MyceliumGlucose2.421 (±0.068)–
Glucose/Propionate1.711 (±0.047)–
Propionate1.961 (±0.081)–
  1. The data are defined as U × mg protein− 1. One unit of enzyme activity was defined as the amount of enzyme producing 1 μmol min− 1 of CoASH under the assay conditions. SD standard deviation; MCS methylcitrate synthase activity
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IMA Fungus

ISSN: 2210-6359

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