Testing spore amyloidity in Agaricales under light microscope: the case study of Tricholoma

IMA Fungus

Table 1 Spore reaction in Melzer’s depending on the method used

Species	CMi H₂0/NH₃/KOH	CM30 H₂0/NH₃/KOH	CM120 H₂0/NH₃/KOH	HM H₂0/NH₃
Amanita argentea	inamyloid	inamyloid	inamyloid	inamyloid
Amanita phalloides	amyloid	amyloid	amyloid	amyloid
Tricholoma acerbum	inamyloid	amyloid	amyloid	amyloid
Tricholoma apium	inamyloid	amyloid	amyloid	amyloid
Tricholoma argyraceum	inamyloid	amyloid	amyloid	amyloid
Tricholoma arvernense	inamyloid	amyloid	amyloid	amyloid
Tricholoma caligatum	inamyloid	amyloid	amyloid	amyloid
Tricholoma colossus	inamyloid	amyloid	amyloid	amyloid
Tricholoma filamentosum	inamyloid	amyloid	amyloid	amyloid
Tricholoma frondosae	inamyloid	amyloid	amyloid	amyloid
Tricholoma fucatum	inamyloid	amyloid	amyloid	amyloid
Tricholoma inamoenum	inamyloid	amyloid	amyloid	amyloid
Tricholoma josserandii	inamyloid	dextrinoid/amyloid	dextrinoid/amyloid	dextrinoid/amyloid
Tricholoma lascivum	inamyloid	amyloid	amyloid	amyloid
Tricholoma orirubens	inamyloid	amyloid	amyloid	amyloid
Tricholoma psammopus	inamyloid	amyloid	amyloid	amyloid
Tricholoma ramentaceum	inamyloid	amyloid	amyloid	amyloid
Tricholoma saponaceum	inamyloid	amyloid	amyloid	amyloid
Tricholoma terreum	inamyloid	dextrinoid/amyloid	dextrinoid/amyloid	dextrinoid/amyloid

CMi spores observed in Melzer’s immediately after squashing and without preheating, CM30 spores observed after leaving the sample in Melzer’s for 30 min and without preheating, CM120 spores observed after leaving the sample in Melzer’s for 120 min and without preheating, HM spores observed in Melzer’s after heating, H₂0/NH₃/KOH soaking media

ISSN: 2210-6359